BioAnalytical Services Laboratory (BAS Lab)

The BioAnalytical Services Laboratory (BAS Lab) provides state-of-the-art sequencing services and instrument availability to promote advances in genomics and molecular biology research. Located at the Institute of Marine and Environmental Technology, BAS Lab services are available to investigators from institutions nationwide.

BASLab services include:

  • DNA Sequencing
  • Plasmid Purification & DNA Sequencing
  • PCR Product Clean-up & DNA Sequencing
  • Genotyping
  • Clone Library Construction

Sample Submission Form (pdf)

BASLabDNA Sequencing
Instrument: ABI 3130 XL Genetic Analyzer, an automated 16 capillary DNA sequencer

  • Technical specifications from Applied Biosystems (pdf)
  • Usual method of analysis: dye terminator
  • Sample Submission Guidelines (pdf)
  • DNA Sequencing Sample Preparation

    Sequence quality will depend largely on the quality of the template. Recommended purification steps for ds DNA include cesium chloride gradient centrifugation, alkaline lysis/PEG precipitation or Qiagen DNA isolation procedures.

Useful DNA Sequencing links:

  • Free EditView software from ABI for viewing and editing chromatograms (Macintosh only, 1.6 MB).
  • Free Chromas software for PCs (allows editing).

BAS Lab Pricing (Current as of October, 2015) 

Services # Samples University &
State of MD Agency
Rate
Commerical Rate
MiSeq 16S Metagenomics
First Sample Run                  
1 $2,200 $4,400
MiSeq 16S/18S/Amplicon Library Prep 2-20 $200 plus the cost of the first run

$400 plus the cost of the first run

MiSeq 16S/18S/Amplicon Library Prep 21-40 $150 plus 50% off the first run

$300 plus 50% off the cost of the first run

MiSeq 16S/18S/Amplicon Library Prep 41-60 $125 plus 75% off your first run

$250 plus 75% off your first run

MiSeq 16S/18S/Amplicon Library Prep              61-80 $125 and first run is included $250 and first run is included
MiSeq 16S/18S/Amplicon Library Prep 81-96 $100 and first run is included $100 and first run is included
MiSeq Whole Genome
First Sample Run
1 $2,000 $4,000
MiSeq Whole Genome Library Prep 2-5 $500 plus the cost of first run

$1000 plus the cost of first run

MiSeq Whole Genome Library Prep 6-10 $400 plus the cost of first run

$800 plus the cost of first run

MiSeq Whole Genome Library Prep 11-15 $350 plus the cost of first run

$350 plus the cost of first run

MiSeq Whole Genome Library Prep 16-96 $350 and first run is included $700 and first run is included
MiSeq Whole Genome Library Prep 96+ $300 and first run is included $600 and first run is included
MiSeq Small RNA Run

12 Please request a quote Please request a quote
MiSeq 300 Cycle Run Only

N/A $2,000 $4,000
MiSeq 500 Cycle Run Only

N/A $2,300 $4,600
MiSeq 600 Cycle Run Only

N/A $2,500 $5,000
DNA Sequencing

1-95 $6 $12
DNA Sequncing

96+ $5 $10
PCR Product Clean Up

1-8 $4 $8
PCR Product Clean Up

9-96 $3 $6
PCR Product Clean Up

96+ $2 $4
Plasmid Prep

1-8 $7 $14
Plasmid Prep

9-96 $6 $12
Plasmid Prep

96+ $5 $10
Genomic DNA Extraction Minimum
10
$20 $40
Genotyping Minimum 96 $6 $12
2100 Bioanalyzer
Price per Run; Customer provides Kit
N/A $80 $160
7500 Real Time PCR

N/A $16 $32
Covaris Ultrasonicator

N/A $25 $50
Fluorchem E

N/A $6 $12
Typhoon

N/A $7 $14


BioAnalytical Services Laboratory Contact Information

BioAnalytical Services Laboratory
Institute of Marine and Environmental Technology
Columbus Center, Room 3001
701 East Pratt Street
Baltimore, MD 21202
Tel: 410-234-8832

Ms. Sabeena Nazar, Laboratory Manager
410-234-8832
sabeena@umces.edu

Dr. Allen Place
, Chair, BASLab Advisory Committee
410-234-8828
place@umces.edu