BioAnalytical Services Laboratory (BASLab)
The BioAnalytical Services Laboratory (BASLab) assists researchers by providing state-of-the-art instrumentation and training to conduct routine analytical, synthetic and separation projects. Although we work primarily with IMET scientists, we also provide services to investigators from other institutions.
BASLab services include:
- DNA Sequencing
- Plasmid Purification & DNA Sequencing
- PCR Product Clean-up & DNA Sequencing
- Genotyping
- Clone Library Construction
Sample Submission Form (pdf)
DNA Sequencing
Instrument: ABI 3130 XL Genetic Analyzer, an automated 16 capillary DNA sequencer
- Technical specifications from Applied Biosystems (pdf)
- Usual method of analysis: dye terminator
- Sample Submission Guidelines (pdf)
- DNA Sequencing Sample Preparation
Sequence quality will depend largely on the quality of the template. Recommended purification steps for ds DNA include cesium chloride gradient centrifugation, alkaline lysis/PEG precipitation or Qiagen DNA isolation procedures.
- Sequencing Protocol (.doc)
- Plasmid Preparation Protocol-96 well plate (.doc)
- PCR Clean-Up Protocol (.doc)
Useful DNA Sequencing links:
- Free EditView software from ABI for viewing and editing chromatograms (Macintosh only, 1.6 MB).
- Free Chromas software for PCs (allows editing).
BASLab Pricing
| Service Type | Rate |
| DNA Sequencing* | $4.00 |
| Plasmid Purification & DNA Sequencing* | $8.00*** |
| PCR Product Clean-up & DNA Sequencing* | $5.50 |
| Genotyping | $7.00 |
| Clone Library Construction** | $1300.00 |
| Extra Handling Charge | $.50/$1.00**** |
*Unedited sequencing.
**Contact BASLab Manager Sabeena Nazar for more information about project options & pricing.
***$8.00 /sample for 96 samples and above. $8.50/sample for 24-95 samples. Minimum 24 samples required.
****if samples are not in compliance with the sample submission guidelines
BioAnalytical Services Laboratory Contact Information
BioAnalytical Services Laboratory
Institute of Marine and Environmental Technology
Columbus Center, Room 3-01
701 East Pratt Street
Baltimore, MD 21202
Tel: 410-234-8832
Ms. Sabeena Nazar, Laboratory Manager
410-234-8832
sabeena@umces.edu
Dr. Allen Place, Chairman, BASLab Advisory Committee
410-234-8828
place@umces.edu

