BioAnalytical Services Laboratory (BAS Lab)

The BioAnalytical Services Laboratory (BAS Lab) provides state-of-the-art sequencing services and instrument availability to promote advances in genomics and molecular biology research. Located at the Institute of Marine and Environmental Technology, BAS Lab services are available to investigators from institutions nationwide.

BASLab services include:

  • DNA Sequencing
  • Plasmid Purification & DNA Sequencing
  • PCR Product Clean-up & DNA Sequencing
  • Genotyping
  • Clone Library Construction

Sample Submission Form (pdf)

BASLabDNA Sequencing

Instrument: ABI 3130 XL Genetic Analyzer, an automated 16 capillary DNA sequencer

Sequence quality will depend largely on the quality of the template. Recommended purification steps for ds DNA include cesium chloride gradient centrifugation, alkaline lysis/PEG precipitation or Qiagen DNA isolation procedures.

Useful DNA Sequencing links:

  • Free EditView software from ABI for viewing and editing chromatograms (Macintosh only, 1.6 MB).
  • Free Chromas software for PCs (allows editing).

BAS Lab Pricing (Current as of August 1, 2014)

Service Type

University & State of MD Agency Rate 

Commercial
Rate

2100 Bioanalyzer $80.00 $160.00
Clone Library Construction $1,500.00 $3,000.00
Genotyping $10.00 $20.00
Qubit Fluorometer $5.00 $10.00
MiSeq RNA Sequencing Service provided Under Quote Only
Genomic DNA Extraction 
(minimum of 10 samples)
$50.00 $100.00
16S Metagenomics First Sample Run $2,200 $4,400
16S Metagenomics Library Prep (sample #2 plus)  $200.00 $400.00

MiSeq Whole Genome Sequencing

$2,000.00 $4,000.00
MiSeq Whole Genome Library (2-3 samples)
$600 $1,200
Typhoon $7.00 $14.00
Plasmid Prep $6.00 $12.00
Flow Cytometry(per 30 minute use) $50.00 $100.00
7500 Real Time PCR $16.00 $32.00
FluorChem E $6.00 $12.00
PCR Clean up $4.00 $8.00
DNA Sequencing $6.00 $12.00
MiSeq 300 Cycle Run Only (New Service) $2,000.00 $4,000.00
Covaris Ultrasonicator $25/sample $50/sample

Volume Rates (pdf)

BioAnalytical Services Laboratory Contact Information

BioAnalytical Services Laboratory
Institute of Marine and Environmental Technology
Columbus Center, Room 3001
701 East Pratt Street
Baltimore, MD 21202
Tel: 410-234-8832

Ms. Sabeena Nazar, Laboratory Manager
410-234-8832
sabeena@umces.edu

Dr. Allen Place
, Chair, BASLab Advisory Committee
410-234-8828
place@umces.edu