BioAnalytical Services Laboratory (BAS Lab)

The BioAnalytical Services Laboratory (BAS Lab) provides state-of-the-art sequencing services and instrument availability to promote advances in genomics and molecular biology research. Located at the Institute of Marine and Environmental Technology, BAS Lab services are available to investigators from institutions nationwide.

BASLab services include:

  • DNA Sequencing
  • Plasmid Purification & DNA Sequencing
  • PCR Product Clean-up & DNA Sequencing
  • Genotyping
  • Clone Library Construction

Sample Submission Form (pdf)

BASLabDNA Sequencing

Instrument: ABI 3130 XL Genetic Analyzer, an automated 16 capillary DNA sequencer

Sequence quality will depend largely on the quality of the template. Recommended purification steps for ds DNA include cesium chloride gradient centrifugation, alkaline lysis/PEG precipitation or Qiagen DNA isolation procedures.

Useful DNA Sequencing links:

  • Free EditView software from ABI for viewing and editing chromatograms (Macintosh only, 1.6 MB).
  • Free Chromas software for PCs (allows editing).

BAS Lab Pricing (Current as of August 1, 2014)

Service Type

University & State of MD Agency Rate 

Commercial
Rate

DNA Sequencing* $6 $12
Plasmid Purification & DNA Sequencing* $12 $24
PCR Product Clean-up & DNA Sequencing* $10 $20
Genotyping $10 $20
Clone Library Construction $1,500 $3,000
MiSeq 16S Metagenomics
1st sample
(Includes library prep and run)
$2,200 $4,400
MiSeq 16S Metagenomics 
Each Additional Samples 2-24 
(Library) 
$200 $400
MiSeq 16S Metagenomics 
Each Additional Samples 25+
(Library) 
Quote Required Quote Required

MiSeq Whole Genome Sequencing
1st Sample 
(Includes library prep and run)

$2,000 $4,000
MiSeq Whole Genome Library, each additional library
$600 $1,200
MiSeq sRNA Sequencing, 6 samples $4,200 $8,400
MiSeq RNA Sequencing Quote Required Quote Required
Flow Cytometry per 1 hour $50 $100
2100 Bioanalyzer $80/chip $160/chip
Quibit Fluorometer $6/sample $12/sample
7500 Real Time PCR $16/hour $32/hour
FluorChem E $6/use $12/use
Typhoon $6/use $6/use

*Unedited sequencing.

BioAnalytical Services Laboratory Contact Information

BioAnalytical Services Laboratory
Institute of Marine and Environmental Technology
Columbus Center, Room 3001
701 East Pratt Street
Baltimore, MD 21202
Tel: 410-234-8832

Ms. Sabeena Nazar, Laboratory Manager
410-234-8832
sabeena@umces.edu

Dr. Allen Place
, Chair, BASLab Advisory Committee
410-234-8828
place@umces.edu