BioAnalytical Services Laboratory (BAS Lab)
The BioAnalytical Services Laboratory (BAS Lab) provides state-of-the-art sequencing services and instrument availability to promote advances in genomics and molecular biology research. Located at the Institute of Marine and Environmental Technology, BAS Lab services are available to investigators from institutions nationwide.
BASLab services include:
- DNA Sequencing
- Plasmid Purification & DNA Sequencing
- PCR Product Clean-up & DNA Sequencing
- Genotyping
- Clone Library Construction
Sample Submission Form (pdf)
DNA Sequencing
Instrument: ABI 3130 XL Genetic Analyzer, an automated 16 capillary DNA sequencer
- Technical specifications from Applied Biosystems (pdf)
- Usual method of analysis: dye terminator
- Sample Submission Guidelines (pdf)
- DNA Sequencing Sample Preparation
Sequence quality will depend largely on the quality of the template. Recommended purification steps for ds DNA include cesium chloride gradient centrifugation, alkaline lysis/PEG precipitation or Qiagen DNA isolation procedures.
- Sequencing Protocol (.pdf)
- Plasmid Preparation Protocol-96 well plate (.pdf)
- PCR Clean-Up Protocol (.pdf)
Useful DNA Sequencing links:
- Free EditView software from ABI for viewing and editing chromatograms (Macintosh only, 1.6 MB).
- Free Chromas software for PCs (allows editing).
BAS Lab Pricing (Current as of July 1, 2012)
| Service Type |
University & State of MD Agency Rate |
Commercial |
| DNA Sequencing* | $5.00 | $10.00 |
| Plasmid Purification & DNA Sequencing* | $10.00 | $20.00 |
| PCR Product Clean-up & DNA Sequencing* | $8.00 | $16.00 |
| Genotyping | $3.00 | $6.00 |
| Clone Library Construction | $1,500.00 | $3,000.00 |
| MiSeq 16S Metagenomics NEW | ||
| 1st sample (Includes library prep and run) |
$1,500 | $3,000.00 |
| Each Additional Sample (Library) up to 24 |
$400 | $800.00 |
*Unedited sequencing.
BioAnalytical Services Laboratory Contact Information
BioAnalytical Services Laboratory
Institute of Marine and Environmental Technology
Columbus Center, Room 3-01
701 East Pratt Street
Baltimore, MD 21202
Tel: 410-234-8832
Ms. Sabeena Nazar, Laboratory Manager
410-234-8832
sabeena@umces.edu
Dr. Allen Place, Chairman, BASLab Advisory Committee
410-234-8828
place@umces.edu