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DTSTART:20191103T020000
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DTSTART:20190310T020000
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UID:calendar.4659.field_date_time.0@www.umces.edu
DTSTAMP:20260403T234148Z
CREATED:20190820T181353Z
DESCRIPTION:September 5\, 2019 3:00pm to 4:00pm    \n    \n      \n\n\n    
 \n\n              \nInstitute of Marine & Environmental Technology\n      
 \n\n\n\n\n  \n\n    \n\n              \n\n\n      \n  \n\n    \n\n        
       \n\n\nTitle: Improving Haematococcus pluvialis growth and astaxanthi
 n production through chemical mutagenesis\n\n\n\nSpeaker: Kia Ramarui (Gra
 duate Student\, UMBC-IMET)\n\n\n\nAbstract: Astaxanthin is a keto-caroteno
 id known for its strong antioxidant activity. It is widely used in the aqu
 aculture and nutraceutical industries. Haematococcus pluvialis has long be
 en used as a natural source of astaxanthin\, however\, its slow growth rat
 e creates a production bottleneck. To overcome this bottleneck\, Haematoco
 ccus mutants with improved growth rate under heterotrophic culture conditi
 ons were generated. This was achieved through screening for mutants with i
 mproved acetate catabolic rates. \n\n\n\n\nAn ethyl methanesulfonate (EMS)
  based chemical mutagenesis approach was used to generate Haematococcus mu
 tants. Two mutants\, YLEMS 3L-33 and KREMS 23D-3\, were identified after t
 hree rounds of screening\, which showed 75% and 21% higher biomass concent
 rations than the WT under heterotrophic growth conditions\, respectively. 
 Interestingly\, when grown under high light to induce astaxanthin biosynth
 esis\, the biomass concentrations of YLEMS 3L-33 and KREMS 23D-3 were 46% 
 and 65% higher than the wild type\, respectively. It was hypothesized that
  these mutants may exhibit a higher acetate catabolism rate under heterotr
 ophic conditions. Acetate metabolism produces acetyl-CoA\, which is an int
 egral intermediate in various biosynthetic pathways related to growth and 
 astaxanthin biosynthesis. Therefore\, the acetate consumption rate\, lipid
  content\, and astaxanthin content of YLEMS 3L-33 and KREMS 23D-3 mutants 
 were comparatively analyzed against the wild type. Our data showed the ace
 tate consumption rate in both mutants was about 60% higher than the wild t
 ype. Additionally\, the final astaxanthin contents of YLEMS 3L-33 and KREM
 S 23D-3 were 80% and 60% higher than the WT\, respectively. The astaxanthi
 n productivities of both mutants were 2.7-fold higher than the WT.\n\n\n\n
 Host: Dr. Yantao Li
DTSTART;TZID=America/New_York:20190905T150000
DTEND;TZID=America/New_York:20190905T160000
LAST-MODIFIED:20190822T160813Z
SUMMARY:IMET Seminar: Kia Ramarui (Graduate Student\, UMBC-IMET)
URL;TYPE=URI:https://www.umces.edu/events/imet-seminar-kia-ramarui-graduate
 -student-umbc-imet
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