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UID:calendar.4692.field_date_time.0@www.umces.edu
DTSTAMP:20260405T191442Z
CREATED:20190826T131145Z
DESCRIPTION:September 4\, 2019 3:30pm    \n    \n      \n\n\n    \n\n      
         \nChesapeake Biological Laboratory\n      \n\n\n\n\n  \n\n    \n\n
               \n\n\n        \n\n    \n\n              \n\n\n\nAnaerobic am
 monium oxidation (anammox) is widely considered as a promising N removal t
 echnology to treat ammonium rich wastewater. In the anammox process\, nitr
 ite (NO2-) is used as an electron acceptor to oxidize ammonium (NH4+) yiel
 ding environmentally inert dinitrogen (N2) gas as the main product. Howeve
 r\, studies showed that NO2- also causes substrate toxicity\, potentially 
 causing the failure of the anammox reactors. In this project\, we investig
 ated the mechanism of NO2- toxicity to anammox process and proposed to uti
 lize nitrate (NO3-) addition to recover anammox reactors failing due to NO
 2- inhibition. The effectiveness of NO3- addition in preventing anammox fa
 ilure was studied in batch- and continuous-fed-experiments in which anammo
 x was purposely subjected to NO2- stress. The results show that higher rec
 overies of performance were obtained with increasing concentrations of add
 ed NO3-\, with the highest recovery of 80% at 0.8 mM NO3- at pH 7.0. In or
 der to study the relationship between proton gradient and the recovery per
 formance by NO3-\, carbonyl cyanide m-chlorophenyl hydrazine (CCCP) was us
 ed to dissipate the proton gradient of anammox bacteria. The results sugge
 sted that reactivation of NO2--inhibited anammox process by NO3- is irresp
 ective of the proton gradient\, supporting a proposed hypothesis that the 
 detoxification mechanism is the NO2-/NO3- antiporter activity encoded by n
 arK present in anammox cells. To further evaluate the feasibility of using
  NO3- to recover failing anammox reactors\, three UASB were used to mimic 
 the system with normal performance and failing reactors with/without NO3- 
 addition\, respectively. A complete performance recovery was obtained with
 in 4 days when a 3-day exposure of 5.0 mM NO3- was applied to a failing sy
 stem. These results collectively show that NO3- addition can salvage a fai
 ling anammox reactor\, thereby minimizing economic loss and assure the saf
 ety of WWTP to the greatest extent.
DTSTART;TZID=America/New_York:20190904T153000
DTEND;TZID=America/New_York:20190904T153000
LAST-MODIFIED:20190829T150045Z
SUMMARY:CBL Seminar: Guangbin Li (UMCP Dept. of Engineering)
URL;TYPE=URI:https://www.umces.edu/events/cbl-seminar-guangbin-li-umcp-dept
 -engineering
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