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X-WR-CALNAME;VALUE=TEXT:UMCES Events
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DTSTART:20191103T020000
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UID:calendar.4858.field_date_time.0@www.umces.edu
DTSTAMP:20260418T060101Z
CREATED:20191021T173843Z
DESCRIPTION:October 24\, 2019 12:00pm to 1:00pm    \n    \n      \n\n\n    
 \n\n              \nInstitute of Marine & Environmental Technology\n      
 \n\n\n\n\n  \n\n    \n\n              \n\n\n      \n  \n\n    \n\n        
       \n\n\nTitle: Diet and Stomach Microbiota of Juvenile Menhaden\, a Ke
 y Forage Filter Feeding Fish Species\n\n\n\nSpeaker: Ammar Hanif (Graduate
  Student\, UMCES-IMET)\n\n\n\nAbstract: Menhaden represent a key filter fe
 eding forage fish species that serves as a trophic link between the plankt
 on and piscivorous predators in the marine environment. Dietary analysis i
 s difficult because >80 % of the stomach content consists of amorphous mat
 erial. DNA metabarcoding\, using the V3-V4 hypervariable region of 16S and
  the V4-V5 hypervariable region of 18S rRNA gene sequences respectively\, 
 has allowed a comprehensive assessment of gut microbiota and diet items. T
 he method was optimized looking at DNA preparation\, comparison of two seq
 uencing methods\, determination of the representation of host DNA\, qualit
 y filtering of sequences\, post-sequence processing and taxonomic identifi
 cation. Stomach microbiota and diet were compared in juvenile Gulf menhade
 n\, Brevoortia patronus\, caught at two locations\, Two Mile Channel and S
 t. Vincent Sound\, in Apalachicola Bay\, FL in May and July\, 2013. The st
 omach microbiota of samples from both locations showed a predominance of P
 roteobacteria\, Chloroflexi\, Bacteroidetes\, Acidobacteria and Actinobact
 eria\, although significant differences in composition at the class level 
 were seen in samples from the different locations. The microbiota from Two
 -mile Channel showed a higher level of taxonomic richness and there was a 
 strong association between the microbiota and sampling location\, correlat
 ing with differences in salinity. Approximately 1050 diet items were ident
 ified\, although significant differences in the species represented were f
 ound in samples from the two locations. Members of the Stramenopile/Alveol
 ate/Rhizaria (SAR) clade account for 66 % representation in samples from T
 wo Mile Channel\, dominated by the diatoms Cyclotella and Skeletonema\, as
  well as the ciliate Oligotrichia. In contrast\, Metazoa (zooplankton) dom
 inate in samples from St. Vincent Sound\, accounting for over 80 % of the 
 reads. These are mainly Acartia copepods. Since ciliates are considered to
  be microzooplankton\, this means there is just over 60 % representation o
 f phytoplankton in samples from Two Mile Channel and over 90 % representat
 ion of zooplankton in samples from St. Vincent Sound. Overall\, we demonst
 rate a wider picture of the diversity of menhaden juvenile diet items that
  supports a picture of menhaden as an environmental sampler.\n\n\n\nHost: 
 Rose Jagus
DTSTART;TZID=America/New_York:20191024T120000
DTEND;TZID=America/New_York:20191024T130000
LAST-MODIFIED:20191021T173843Z
SUMMARY:IMET Seminar: Ammar Hanif (Graduate Student\, UMCES-IMET)
URL;TYPE=URI:https://www.umces.edu/events/imet-seminar-ammar-hanif-graduate
 -student-umces-imet
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