Seminar: Dr. Shiladitya DasSarma, UMB-IMET

April 11, 2018 3:00pm to 4:00pm iCal Google Calendar

Title: "Molecular genetic analysis and biomedical applications of haloarchaeal virus-like nanoparticles"

Speaker: Dr. Shiladitya DasSarma, UMB-IMET

Abstract: Halophilic Archaea such as Halobacterium sp. NRC-1 produce large quantities of hollow, buoyant, virus-
like nanoparticles called gas vesicles (or GVNPs) (1). GVNPs are extremely stable, proteinaceous, non-
toxic, self-adjuvanting, and easily purified after hypotonic lysis of the salt-loving microorganisms. A
tightly organized gene cluster on a large plasmid, gvpMLKIJHGFFEDACNO, was implicated in their
biogenesis through extensive molecular genetic analysis. Two proteins, one amyloid-like (GvpA) and the
other soluble (GvpC), were shown to constitute major structural components of the nanoparticle, and at
least five additional proteins serve as minor structural or assembly components. Foreign sequences
inserted at the C-terminal region of GvpC resulted in their display on the nanoparticle surface (2,3). An
improved genetic system has recently been developed to allow for a more facile display of foreign
sequences, including antigenic and therapeutic peptides and proteins (4-7). Displayed antigenic proteins
include envelope proteins, enzymes, and extracellular secreted proteins followed by testing of
protection using disease models in several cases. The source of foreign proteins displayed on the surface
of GVNPs thus far include antigens from the simian immunodeficiency virus (SIV) (3), a facultative
intracellular pathogen, Salmonella enterica (5), and the parasitic protozoan Plasmodium spp. (6), as well
an innate immune system protein from mice (7). Most recently, we also established that GVNPs may be
delivered by microneedle administration (8), further expanding the versatility of the system for
biomedical applications. 

References: 1. Vaccines 3:686-702, 2015; 2. Journal of Biotechnology 88:119-128; 3. Journal of
Biotechnology 114:225-237, 2004; 4. BMC Biotechnology 13:112, 2013; 5. Vaccine 32:4543-4549, 2014;
6. Malaria Journal 14:406, 2015; 7. Scientific Reports 6:33679, 2016; 8. Molecular Pharmaceutics 14:953-
958, 2017.

Host: Dr. Yantao Li