Zeiss AxioPhot Fluorescent Microscope

Our other large Zeiss epifluorescent microscope is the Zeiss Axiophot. In addition to fluorescence microscopy, this microscope is also used for phase contrast and differential interference contrast (DIC) microscopy.  Though it is an older scope, it was recently outfitted with a modern digital Zeiss AxioCam MR camera and utilizes the same Zen software as the new Axio Imager 2. Phase contrast and DIC microscopy allow for observation of unstained, live cells and structures. For fluorescent microscopy, this microscope utilizes a Zeiss XBO 75 (75W xenon arc lamp) and HBO 100 (100W mercury arc lamp).

For additional information including manuals, manufacturer links, protocols and publications, see our resources page.  To see images captured by this microscope, see the gallery.

Equipment Specifications:
	LIGHT SOURCE:		Zeiss XBO-75/HBO-100
	CAMERA:		Zeiss AxioCam MR
	OBJECTIVES:		100x oil, 63x oil, 40x Ph1, 10x Ph2
	ATTACHMENT(S):		Phase Contrast and DIC capabilities
	COMPUTER:		Dell Optiplex 7010
	SOFTWARE:		Zen 2012
Filter Cube Specifications:
	ID	EXCITATION	BEAM SPLITTER	EMISSION
	02 (opens in a new tab)	G 365 (opens in a new tab)	FT 395 (opens in a new tab)	LP 420 (opens in a new tab)
	05 (opens in a new tab)	BP 395-440 (opens in a new tab)	FT 460 (opens in a new tab)	LP 470 (opens in a new tab)
	09 (opens in a new tab)	BP 450-490 (opens in a new tab)	FT 510 (opens in a new tab)	LP 515 (opens in a new tab)
	15 (opens in a new tab)	BP 546/12 (opens in a new tab)	FT 580 (opens in a new tab)	LP 590 (opens in a new tab)
	38	BP 470/40	FT 495	BP 525/50